Anti-BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1

Anti-BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1

• Background: BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1)controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Solanum lycopersicum
• Not reactive in: Hordeum vulgare, Lactuca sativa, Nicotiana benthamiana, Oryza sativa
• Immunogen: KLH-conjugated peptide, chosen fromArabidopsis thalianaBak1 sequence, TAIR:AT4G33430UniProt:Q94F62
• Clonality: Polyclonal
• Applications: Immunoprecipitation (IP), Western blot (WB)
• Dilution: 2 µl/50 µl of Protein G agarose, 1 : 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 68 | 70 kDa
• Precautions: Extra Information on CE extraction buffer:CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na2MoO4and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases.  Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.
• References & Citations: Leeet al. (2024). Reprogramming of flagellin receptor responses with surrogate ligands. Nat Commun. 2024 Nov 12;15(1):9811.doi: 10.1038/s41467-024-54271-5.Jinget al. (2024). Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis. Nat Commun. 2024 Mar 8;15(1):2028.doi: 10.1038/s41467-024-46289-6.Xuet al. (2022) The Phloem Intercalated With Xylem-Correlated 3 Receptor-Like Kinase Constitutively Interacts With Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 and Is Involved in Vascular Development in Arabidopsis. Front Plant Sci. 2022 Jan 11;12:706633. doi: 10.3389/fpls.2021.706633. PMID: 35087541; PMCID: PMC8786740.Kalischuket al. (2022) Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants. Commun Biol. 2022 May 25;5(1):497. doi: 10.1038/s42003-022-03439-0. PMID: 35614138; PMCID: PMC9132969.KatarzynaParys et al (2021) Signatures of antagonistic pleiotropy in a bacterial flagellin epitope,Cell Host & Microbe,Volume 29, Issue 4,2021,Pages 620-634.e9,ISSN 1931-3128,https://doi.org/10.1016/j.chom.2021.02.008.(https://www.sciencedirect.com/science/article/pii/S1931312821000871)Zhanget al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Huet al. (2018). A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis. Nat Plants. 2018 Apr;4(4):205-211. doi: 10.1038/s41477-018-0123-z. (CoIP)
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.