Anti-BRI1 | Brassinosteroid insensitive 1

Anti-BRI1 | Brassinosteroid insensitive 1

• Background: BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1)is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2,BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: Hordeum vulgare, Oryza sativa, Solanum lycopersicum
• Immunogen: KLH-conjugated synthetic peptide derived fromArabidopsis thalianaBRI1 protein, Uniprot:O22476, TAIR:AT4G39400
• Clonality: Polyclonal
• Applications: Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1 : 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: above 130 kDa (due to N-glycosylation)
• Precautions: Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans.For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.
• References & Citations: Kamińskaet al. (2024). Comprehensive elucidation of the differential physiological kale response to cytokinins under in vitro conditions. BMC Plant Biol. 2024 Jul 15;24(1):674.doi: 10.1186/s12870-024-05396-8.Jinget al. (2024). Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis. Nat Commun. 2024 Mar 8;15(1):2028.doi: 10.1038/s41467-024-46289-6.Ormanceyet al. (2023) Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nat Commun. 2023;14(1):254. Published 2023 Jan 17. doi:10.1038/s41467-023-35951-0.Luo, Takagi, Claus LAN, et al. (2023) Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1. EMBO Rep. 2022;23(4):e53354. doi:10.15252/embr.202153354Leeet al (2021). Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Chenet al. (2019). BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana. Nat Commun. 2019 Sep 13;10(1):4164. doi: 10.1038/s41467-019-12118-4.Houet al. (2019). Less Conserved LRRs Is Important for BRI1 Folding. Front Plant Sci. 2019 May 21;10:634. doi: 10.3389/fpls.2019.00634. eCollection 2019.Chenet al. (2019). BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis. Mol Plant. 2019 Jun 20. pii: S1674-2052(19)30207-2. doi: 10.1016/j.molp.2019.06.006.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.