Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a

• Background:

  RPT2a (Regulatory particle triple-A ATPase subunit 2a)is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation.Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name(s): 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.

• CAS Number:

  9007-83-4

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Recombinant, full-lenght RPT2a ofArabidopsis thalianaRPT2a protein sequence UniProt:Q9SZD4-1, TAIR:At4g29040overexpressed inE.coli, purified from a gel piece

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 3000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl, of sterile water

• Molecular Weight:

  49.4 | 50 kDa

• Precautions:

  This antibody is also recognizing RPT2b protein.Recommended western blot conditions:SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to:Smalleet al. (2002).

• References & Citations:

  Meng,Wang, Hao, et al. (2023) RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis. Plant Physiol. 2023;191(1):446-462. doi:10.1093/plphys/kiac511Smalleet al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.