Anti-RPN5a | 26S proteasome regulatory subunit, putative (RP

CAT:

451-AS19 4265

Size:

50 µL

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Anti-RPN5a | 26S proteasome regulatory subunit, putative (RP

Background: RPN5a | 26S proteasome regulatory subunit, putative (RPN5)is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.
CAS Number: 9007-83-4
Host: Rabbit
Reactivity: Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Immunogen: Recombinant RPN5a ofArabidopsis thalianaUniProt:F4KFD7-1, TAIR:At5g09900,overexpressed inE.coli, purified from a gel piece
Clonality: Polyclonal
Applications: Western blot (WB)
Dilution: 1 : 5000 (WB)
Purity: Serum
Format: Lyophilized
Reconstitution: For reconstitution add 50 µl, of sterile water
Molecular Weight: 52.7 | 50 kDa
Precautions: This antibody is also recognizing RPN5b.Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.
References & Citations: Xieet al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.Smalleet al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Storage Conditions: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

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