Anti-14-3-3 GRF | General regulatory element

• Background:

  GRF (General regulatory factor 2)or14.3.3s are 30 kDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.

• Host:

  Rabbit

• Reactivity:

  Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum, Neochloris oloabundans (algae), Oryza sativa, Picrorhiza kurroa, Phaseolus vulgaris, Panax notoginseng, Zea mays, Vicia faba

• Not reactive in:

  This antibody does not bind to 14-3-3-like protein D ofNicotiana tabacum.

• Immunogen:

  Conserved surface-exposed peptide conjugated toKLH.This sequence is repeated in all 12 “canonical” members of theArabidopsis thalianaGRF/14-3-3 family. GRF1 GF14 chi UniProt:F4JJ94, TAIR:AT4G09000, GRF2 GF14 omega, UniProt:Q01525, TAIR:AT1G78300, GRF3 GF14 psi UniProt:F4KBI7, TAIR:AT5G38480, GRF4 GF14 phi UniProt:F4HWQ5, TAIR:AT1G35160, GRF5 GF14 upsilon UniProt:P42645, TAIR:AT5G16050, GRF6 GF14 lambda UniProt:P48349#P48349-2, TAIR:AT5G10450, GRF7 GF14 nu UniProt:Q96300, TAIR:AT3G02520, GRF8 GF14 kappa UniProt:F4KHY7, TAIR:AT5G65430, GRF9 GF14 mu UniProt:F4IP55, TAIR:AT2G42590, GRF10 GF14 epsilon UniProt:F4I1C1, TAIR:AT1G22300, GRF11 GF14 omicron UniProt:Q9S9Z8, TAIR:AT1G34760, GRF12 GF14 iota UniProt:Q9C5W6TAIR:AT1G26480.

• Clonality:

  Polyclonal

• Applications:

  Immunoprecipitation (IP), Western Blot (WB)

• Dilution:

  1: 2000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  20-28 kDa (depending upon an isoform)

• Precautions:

  This product can be sold containing proclin if requested

• References & Citations:

  Liet al (2023) Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1Franziskaet al. (2022) Auxin application to maize plants at flowering increases abundance and activity of plasma membrane H+-ATPase in developing maize kernels, Journal of Plant Nutrition and Soil Science, Volume 185, Issue 5, October 2022, https://doi.org/10.1002/jpln.202200155.Guoet al. (2022) Acetylproteomics analyses reveal critical features of lysine-?-acetylation in Arabidopsis and a role of 14-3-3 protein acetylation in alkaline response. Stress Biology 2, 1 (2022). https://doi.org/10.1007/s44154-021-00024-z. (immunoprecipitation)Kumariet al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.Dongxuet al. (2020). Magnesium reduces cadmium accumulation by decreasing the nitrate reductase-mediated nitric oxide production in Panax notoginseng roots. Journal of Plant Physiology. Available online 7 February 2020, 153131Guptaand Shaw (2020). Biochemical and molecular characterisations of salt tolerance components in rice varieties tolerant and sensitive to NaCl: the relevance of Na+ exclusion in salt tolerance in the species . Funct Plant Biol. 2020 Jul 30.doi: 10.1071/FP20089Pertl-Obermeyeret al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480.Obroucheva(2017). Participation of Plasma Membrane H+-ATPase in Seed Germination. Internat. J. of Cell Science & Molecular Biol. Vol. 2 Issue 3. DOI : 10.19080/IJCSMB.2017.02.555589.Barklaet al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4