Anti-mCherry (rabbit antibody)

• Background:

  MCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer) . The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals) .

• HS Code:

  38221900

• Host:

  Rabbit

• Reactivity:

  MCherry

• Immunogen:

  Recombinant full length mCherry expressed and purified from E. coli.

• Clonality:

  Polyclonal

• Applications:

  Immunocytochemistry (ICC), Western blot (WB)

• Field of Research:

  BFP/FITC/GFP/RFP/TRITC/YFP

• Dilution:

  1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

• Purity:

  Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Precautions:

  In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.

• References & Citations:

  Song et al. (2024) . Substrate-induced condensation activates plant TIR domain proteins.Nature. 2024 Mar 13.doi: 10.1038/s41586-024-07183-9.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  MScarlett

• CAS Number:

  9007-83-4