Anti-GST-tag (rabbit antibody, polyclonal)

Anti-GST-tag (rabbit antibody, polyclonal)

• Background: GST-tag(glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
• CAS Number: 9007-83-4
• Host: Rabbit
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: GST protein
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Western blot (WB)
• Dilution: 1: 1: 1000 (ELISA), 1: 2000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 26 kDa
• Precautions: Overnight incubation of this antibody with dilution 1:5 000 with 0,5 µg of GST-tagged protein produced strong background but works fine with samples of totalEscherichia colilysate if GST-tagged protein is expressed in low amounts.
• Storage Conditions: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.