Anti-ECA1 | Calcium-transporting ATPase 1, endoplasmic retic

Anti-ECA1 | Calcium-transporting ATPase 1, endoplasmic retic

• Background: Calcium-transporting ATPase 1, endoplasmic reticulum-typeisamagnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. The enzyme can also transport zinc. Alternative names: ACA3, ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated peptide derived fromArabidopsis thalianaECA1 sequence, cytosolic loop, between TM4 and TM5, UniProt:P92939, TAIR:At1g07810
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 10 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 116.3 kDa
• Precautions: The protein shows tissue specific expression, shown on a blot included inWuet al. (2000). Lowest amounts of ECA1 are found in siliques.
• References & Citations: Wuet al. (2000). An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress. Plant Physiol. 2002 Sep;130(1):128-37.Lianget al. (1997). ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8579-84.
• Storage Conditions: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.