Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody)

Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody)

• Background: The Plasma MembraneH+ATPaseis a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
• Host: Rabbit
• Reactivity: Actinidia chinensis, Aesculus hippocastanum,Arabidopsis thaliana, Camellia sinensiscv. Shu-chazao,Chara australisR.Br,Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh.c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata(fern), Ricinus communis, Salicornia bigelovii, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Vicia faba, Zea mays
• Not reactive in: Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba
• Immunogen: KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences includingArabidopsis thalianaATPase 1 (UniProt:P20649, TAIR:At2g18960) and ATPase 2 (UniProt:P19456, TAIR:At4g30190), 3 (UniProt:P20431, TAIR:At5g57350), 4 (UniProt:Q9SU58, TAIR:At3g47950), 6 (UniProt:Q9SH76, TAIR:At2g07560), 7 (UniProt:Q9LY32, TAIR:At3g60330), 8 (UniProt:Q9M2A0, TAIR:At3g42640), 9 (UniProt:Q42556, TAIR:At1g80660), 11 (UniProt:Q9LV11, TAIR:At5g62670) ofArabidopsis thalianaand hydrogen ATPase ofChlamydomonas reinhardtii(Q9FNS3)
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
• Dilution: 1 : 600-1 : 1000 (IF), 1 : 100 (IL), 1 : 1000-1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 90-95 kDa (Arabidopsis thaliana,depending upon an isoform)
• Precautions: VERY IMPORTANT:Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot.Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates.H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents.This product can be sold containing ProClin if requested.
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• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.