Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase

Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase

• Background: Plant vacuole V-ATPaseis responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase.Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margaritaSwingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh.c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum,Noccaea caerulescens, Oryza sativa, Petunia hybridacv. Mitchell, Populussp., Pteris vittata (fern), Salicornia bigelovii,Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera
• Not reactive in: Avicenniasp., mangrove plants,Schizosaccharomyces pombe
• Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase includingArabidopsis thalianaUniProt:Q39258-1, TAIR:At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 26 | 31 kDa (Arabidopsis thaliana)
• Precautions: V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be foundhere.
• References & Citations: Salazaret al. (2024). SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii. Nat Commun. 2024 May 20;15(1):4279.doi: 10.1038/s41467-024-48595-5.Collinset al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172Skalickyet al. (2021) Auxin Metabolite Profiling in Isolated and Intact Plant Nuclei. Int J Mol Sci. 2021 Nov 16;22(22):12369. doi: 10.3390/ijms222212369. PMID: 34830250; PMCID: PMC8620152.Roustanet al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.Prinsiet al. (2020). Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020 Feb 6;21(3). pii: E1076. doi: 10.3390/ijms21031076.Kuanget al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Pertl-Obermeyeret al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480.Migockaet al. (2018). Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess. Plant J. 2018 Jun 22. doi: 10.1111/tpj.14006.Zhuet al. (2018). A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function. Hortic Res. 2018 Feb 7;5:6. doi: 10.1038/s41438-017-0014-x.Lynchet al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730.Nagelet al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Vera-Estrellaet al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Xinget al. (2016). Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS One. 2016 Dec 19;11(12):e0168467. doi: 10.1371/journal.pone.0168467.LaMontagneet al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.Barklaet al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.Liuet al. (2016). iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016 Sep 2;146:80-9. doi: 10.1016/j.jprot.2016.06.031.Wattelet-Boyeret al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Jiskrovaet al. (2016). Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots. N Biotechnol. 2016 Jan 8. pii: S1871-6784(16)00002-9. doi: 10.1016/j.nbt.2015.12.010Langet al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.