Anti-RbcL II | Rubisco large subunit, form II

Anti-RbcL II | Rubisco large subunit, form II

• Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase)catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms.Form II Rubisco is present in many photosynthetic bacteria and archaea and in some photosynthetic dinoflagellates.The large subunit (LSU) of form I Rubisco is encoded by the RbcL/cbbL gene, while form II LSU is encoded by cbbM.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum
• Not reactive in: Burkholderi,Cyanobiumsp.
• Immunogen: KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1:10 000 (IF), (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 51.7 kDa
• Precautions: Protein extraction from diatoms protocol can be foundhere.
• References & Citations: Choet al. (2021). SxtA localizes to chloroplasts and changes to its 3'UTR may reduce toxin biosynthesis in non-toxic Alexandrium catenella (Group I). Harmful Algae, 2021,101972,ISSN 1568-9883, https://doi.org/10.1016/j.hal.2020.101972. ImmunolocalizationBauschet al. (2019). Combined effects of simulated acidification and hypoxia on the harmful dinoflagellate Amphidinium carterae. Marine Biology, June 2019, 166:80.Longet al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.