Anti-RbcL | Rubisco large subunit, form I (chicken)

Anti-RbcL | Rubisco large subunit, form I (chicken)

• Background: Rubisco(Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
• CAS Number: 9007-83-4
• Host: Chicken
• Reactivity: Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa,mixed phytoplankton,Microcystis aeruginosaPCC7806,Pinus strobus,Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcussp. PCC7842,Thiobacillussp.Ulmussp.
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, includingArabidopsis thalianaUniProt:O03042, TAIR:AtCg00490,Synechococcussp.Q3ALL1
• Clonality: Polyclonal
• Applications: Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)
• Dilution: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 µg of total cellular protein, (WB)
• Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
• Format: Liquid
• Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
• Precautions: This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, (AS03 037) combined with Rubisco ready to use standard (AS01 017) is recommended.
• References & Citations: Guljamowet al. (2021) Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806. Microorganisms. 2021 Jun 10;9(6):1265. doi: 10.3390/microorganisms9061265. PMID: 34200971; PMCID: PMC8230624. (IF)Morinet al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1–20. (sea-ice diatom)Lvet al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gellértet al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005.Robertet al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.Morashet al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043.MacKenzieet al. (2005). Inorganic carbon acclimation in Synechococcus elongatus alters the dynamics of macromolecular pooks and photosynthetic fluxes in response to increased light. Photosynt Research 85: 341-357.Schofieldet al. (2003). Changes in macromolecular allocation in nondividins algal symbionts allow for photosynthetic acclimation in the lichen Lobaria pulmonaria. New Phytol 159: 709-718.
• Storage Conditions: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.