Anti-RbcL | Rubisco large subunit, form I (rabbit)

Anti-RbcL | Rubisco large subunit, form I (rabbit)

• Background: This antibody is especially suitable for quantifying of Rubisco in plant and algal samples.Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Agostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata (benthic foraminifer), Beta vulgaris, Begoniasp.,Bienertia sinuspersici, Brassica napus, Kandelia candel, Cannabis sativaL.,  Chaetoceros furcellatus,Chlorococcum dorsiventrale,Colobanthus quitensis, Cicer arietinum, Chenopodium quinoa, Chlamydomonas raudensis, Chlamydomonas reinhardtii,Colobanthus quitensisKunt Bartl,  Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus,Cyanophora paradoxa, Cylindrospermopsis raciborskiiCS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margaritaSwingle, Fraxinus mandshurica,Fucus vesiculosus, Gladieria sulphuraria,Glycine max, Gonyaulax polyedra, Gongolaria barbata, Guzmaniahybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis(C.C.Davis) s) G.Hansen & Ø.Moestrup (Wilson isolate), Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybridacv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis,, Porphyra sp., Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharumsp., Schima superba, Skeletonema costatum(diatom), Skeletonema marinoi (diatom), Solanum lycopersicum,Spinacia oleracea,lichens, Stanleya pinnata, Symbiodinium sp., Synechococcus PCC 7942, Synechococcus elongatusUTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus sp.(surface and deep water ecotype), Triticum aestivum,dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotrophMethylacidiphilum fumariolicumstrain SolV,Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and cyanobacterial RbcL protein sequences (form I L8S8 and form II L2), including,Arabidopsis thalianaO03042,Hordeum vulgareP05698,Oryza sativaP0C510,Chlamydomonas reinhardtiiP00877,SynechococcusPCC 7920A5CKC5
• Clonality: Polyclonal
• Applications: Immunofluorescence/confocal Immunolocalization (IL) (IF), Immunogold (IG), Tissue Printing (TP), Western blot (WB)
• Dilution: Immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images seePrinset al. (2008), detailed protocol available on request,  1: 800 (TP), 1: 5000 - 10 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
• Precautions: This antibody was used in:Immunocytochemical staining of diatoms according to Schmid (2003) J Phycol 39: 139-153 and Wordemann et al. (1986) J Cell Biol 102: 1688-1698.ImmunofluorescenceDreieret al. (2012). FEMS Microbial Ecol., March 2012.Western blot and tissue printing during a student courseMaet al. (2009).As a loading controlSunet al. (2020).Protocol for Rubisco quantification using this antibody can be foundhere.
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• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.