Anti-SAG12 | Senescence-specific cysteine protease SAG12

• Background:

  Senescence-specific cysteine protease (SAG12)is a cysteine protease influenced by cytokinin, auxin and sugars and involved in developmental senescence specific cell death.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Brassica napus, Hordeum vulgare

• Not reactive in:

  Citrus sinensis

• Immunogen:

  KLH-conjugated peptide derived fromArabidopsis thalianaSAG12 protein sequence, UniProt:Q38886, TAIR:AT5G45890

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 2000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  38 | 28 kDa

• Precautions:

  Recommended plant extraction buffer: SeeDurianet al. (2019). Denature in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) at 70◦C for 5 min

• References & Citations:

  Durianet al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Durianet al. (2019). PROTEIN PHOSPHATASE 2A-B'? controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Franket al. (2019). The Hordeum vulgare cysteine protease HvPAP14 plays a role in degradation of chloroplast proteins. J Exp Bot. 2019 Aug 12. pii: erz356. doi: 10.1093/jxb/erz356.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4