Goat anti-Dog IgG Fc specific, Unconjugated

Goat anti-Dog IgG Fc specific, Unconjugated

• Background: Goat anti-Dog IgG Fc, Unconjugatedis a secondary antibody which binds to dog IgG FC part in immunological assays.
• CAS Number: 9007-83-4
• Host: Goat
• Reactivity: Dog IgG, Fc part
• Not reactive in: The antiserum does not cross-react with any other component of the dog immunoglobulin system
• Immunogen: Purified dog IgG from pooled dog serum
• Clonality: Polyclonal
• Applications: Immuniprecipitation (IP)
• Dilution: Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
• Purity: Protein G purified goat IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1 ml of sterile water
• Precautions: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details.No preservative added, as it may interfere with the antibody activity.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.