Anti-CPN60A1 | Chaperonin 60 subunit alpha 1 (chloroplastic)

Anti-CPN60A1 | Chaperonin 60 subunit alpha 1 (chloroplastic)

• Background: CPN60 alpha 1 (chaperonin 60 subunit alpha 1, chloroplastic)binds small and large subunits of Rubisco. Assists in protein folding and required for proper chloroplast development. Synonymes: protein SCHLEPPERLESS, RuBisCO large subunit-binding protein subunit alpha 1.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana,Arabidopsis thalianacell culture,Cicer arietinum,Nicotiana tabacum,Phaseolus vulgaris,Pisum sativm,Zea mays
• Not reactive in: Cyanobacteria, algae
• Immunogen: KLH-conjugated synthetic peptide derived from known CPN60 sequences, includingArabidopsis thalianaUniProtP21238. TAIRAT2G28000. The peptide is conserved in chloroplastic CPN60A1 but NOT the close relative CPN60A2.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 57.1 kDa (mature protein)
• References & Citations: Muet al. (2024). Plastid HSP90C C-terminal extension region plays a regulatory role in chaperone activity and client binding.Plant J. 2024 Jul 5.doi: 10.1111/tpj.16917.Lanet al (2023). UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescence.Singh, Muthamilarasan, Prasad (2022). SiHSFA2e regulated expression of SisHSP21.9 maintains chloroplast proteome integrity under high temperature stress. Cell Mol Life Sci. 2022;79(11):580. Published 2022 Nov 3. doi:10.1007/s00018-022-04611-9.Liet al. (2022) The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants. Cell Rep. 2022 Apr 12;39(2):110664. doi: 10.1016/j.celrep.2022.110664. PMID: 35417702.Jeranet al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Jianget al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399.Dograet al. (2019). Impaired PSII proteostasis triggers an UPR-like response in the var2 mutant of Arabidopsis thaliana. J Exp Bot. 2019 Apr 16. pii: erz151. doi: 10.1093/jxb/erz151.Landeet al. (2019). Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses. Plant Physiology and Biochemistry Volume 146, January 2020, Pages 337-348.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.