Anti-RbcS | Rubisco small subunit (SSU)

Anti-RbcS | Rubisco small subunit (SSU)

• Background: Rubiscocatalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtii, Cucumis sativus, Cucurbita pepo, Cyanthobasis fruticulosa, Hordeum vulgare, Malus domestica, Nicotiana tabacum, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Solanum lycopersicum
• Not reactive in: Cyanobacteria
• Immunogen: KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1A UniProt:P10795,TAIR:AT1G67090, and 1B ofArabidopsis thalianaUniProt:P10796At5g38430
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 5000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 20 | 15 kDa
• Precautions: This product can be sold containing ProClin if requested
• References & Citations: Limet al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Mazuret al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Bernauet al. (2021) Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins,Journal of Chromatography A,Volume 1652,2021,462379,ISSN 0021-9673,https://doi.org/10.1016/j.chroma.2021.462379. (https://www.sciencedirect.com/science/article/pii/S0021967321005033)Maet al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.Akmoucheet al. (2019). Do nitrogen- and sulphur-remobilization-related parameters measured at the onset of the reproductive stage provide early indicators to adjust N and S fertilization in oilseed rape (Brassica napus L.) grown under N- and/or S-limiting supplies? Planta. 2019 Dec;250(6):2047-2062. doi: 10.1007/s00425-019-03284-2.Karimzadeganet al. (2018). The Effect of Methyl Jasmonate and Temperature on the Transient Expression of Recombinant Proteins in Cucurbita pepo L. Mol Biotechnol. 2018 Nov 27. doi: 10.1007/s12033-018-0138-8.Luanet al. (2018). Elucidating the hypoxic stress response in barley (Hordeum vulgare L.) during waterlogging: A proteomics approach. Sci Rep. 2018 Jun 25;8(1):9655. doi: 10.1038/s41598-018-27726-1.Shanmugabalajiet al. (2018). Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018 Aug 20;28(16):2616-2623.e5. doi: 10.1016/j.cub.2018.06.006.Raviet al. (2018). Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. Int J Mol Sci. 2018 Feb 16;19(2). pii: E585. doi: 10.3390/ijms19020585.Hartingset al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401.Yinet al. (2016). Interplay between mitogen-activated protein kinase and nitric oxide in brassinosteroid-induced pesticide metabolism in Solanum lycopersicum. J Hazard Mater. 2016 Oct 5;316:221-31. doi: 10.1016/j.jhazmat.2016.04.070. Epub 2016 Apr 29.Robertet al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.Dahalet al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.Krasuskaet al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.Huanget al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185–194.Kimet al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.Sunet al. (2014). The response of rbcL, rbcS and rca genes in cucumber, (Cucumis sativus L.) to growth and induction light intensity. Acta Physiol Plant, October 2014, Volume 36, Issue 10, pp 2779-2791
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.