Anti-Sec21p | Gamma subunit, COP vesicles

Anti-Sec21p | Gamma subunit, COP vesicles

• Background: Sec21pis a constituent of the COPI vesicle coatomer.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
• Not reactive in: Nicotiana tabacum, Microsporidiasp.
• Immunogen: GST fusion of a part of recombinant Sec21 ofArabidopsis thalianaQ0WW26,At4g34450
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1 : 1000 (IF), 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 98 kDa
• Precautions: This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimplet al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaleret al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
• References & Citations: Vogelet al. (2022) Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking. Nat Commun. 2022;13(1):6897. Published 2022 Nov 12. doi:10.1038/s41467-022-34637-5.Brumm, Singh, Kriechbaum, et al. (2022) N-terminal domain of ARF-GEF GNOM prevents heterodimerization with functionally divergent GNL1 in Arabidopsis. Plant J. 2022;112(3):772-785. doi:10.1111/tpj.15980.Skalickyet al. (2021) Auxin Metabolite Profiling in Isolated and Intact Plant Nuclei. Int J Mol Sci. 2021 Nov 16;22(22):12369. doi: 10.3390/ijms222212369. PMID: 34830250; PMCID: PMC8620152.Hurnyet al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Lupetteet al. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research Volume 38, March 2019, 101415.Singhet al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.Kitakuraet al. (2017). BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. doi: 10.1093/pcp/pcx118.Nagelet al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Wanget al. (2016). Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis. J Exp Bot. 2016 Dec;67(22):6323-6335. Epub 2016 Oct 27.Wattelet-Boyeret al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshireet al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Tanakaet al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization).Hopffet al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24.Pimplet al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.