Donkey anti-Rabbit IgG (H&L), DyLight® 633 conjugated

Donkey anti-Rabbit IgG (H&L), DyLight® 633 conjugated

• Background: Donkey anti-Rabbit IgG (H&L) - DyLight® 633 Conjugatedis a secondary antibody conjugated toDyLight® 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
• CAS Number: 9007-83-4
• Host: Donkey
• Immunogen: Purified Rabbit IgG, whole molecule
• Clonality: Polyclonal
• Dilution: 1 : 20-1 : 2000 for most applications
• Purity: Immunogen affinity purified donkey IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
• Storage Conditions: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.