Donkey anti-Rabbit IgG (H&L), HRP conjugated

Donkey anti-Rabbit IgG (H&L), HRP conjugated

• Background: Donkey anti-rabbit IgG (H&L)is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) inimmunological assays.
• CAS Number: 9007-83-4
• Host: Donkey
• Reactivity: Rabbit IgG (H&L)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Rabbit IgG
• Clonality: Polyclonal
• Applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
• Dilution: The optimal working dilution should be determined by the investigator
• Purity: Immunogen affinity purified donkey IgG.
• Format: Lyophilized
• Precautions: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
• References & Citations: Cooket al. (2024). Chloroplast phosphatases LPPγ and LPPε1 facilitate conversion of extraplastidic phospholipids to galactolipids. Plant Physiol. 2024 May 31;195(2):1506-1520. doi: 10.1093/plphys/kiae100.
• Storage Conditions: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.