Donkey anti-Rabbit IgG (H&L), ALP conjugated

• Background:

  Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.

• HS Code:

  38221900

• Host:

  Donkey

• Reactivity:

  Rabbit IgG (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Rabbit IgG

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

• Field of Research:

  Anti-Rabbit

• Dilution:

  1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), , 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Liquid

• Precautions:

  This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

• Additionnal Information:

  ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

• References & Citations:

  Kay et al. (2014) . Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• Prediction Reactivity:

  Rabbit IgG (H&L)

• CAS Number:

  9007-83-4