Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, DyLight® 550 conjugated,min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

• Background:

  Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight®550 Conjugate, min. cross-reactivity to bovine, human, or mouse lgG or serum proteinsis a secondary antibody conjugated toDyLight® 550, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays.DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• Host:

  Goat

• Immunogen:

  Purified rabbit IgG, whole molecule

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• Precautions:

  Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.

• References & Citations:

  Provostet al. (2019). Sci Rep. 2019 Nov 29;9(1):17967. doi: 10.1038/s41598-019-53528-0.

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4