Goat anti-Rabbit IgG (H&L), ALP conjugated

• Background:

  Goat anti-rabbit IgG (H&L)is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins inimmunological assays.

• Host:

  Goat

• Reactivity:

  Rabbit IgG heavy and light chains (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Rabbit IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  1 : 500-1: 8 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 500-1: 8 000 (WB)

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Liquid, clear, colorless.

• Precautions:

  Based upon IEP, this antibody binds to:• Heavy chains on rabbit IgG• Light chains on all rabbit immunoglobulinsNo reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

• References & Citations:

  Rodrigueset al (2022) Exploring the Applicability of Calorespirometry to Assess Seed Metabolic Stability Upon Temperature Stress Conditions-Pisum sativum L. Used as a Case Study. Front Plant Sci. 2022 Apr 27;13:827117. doi: 10.3389/fpls.2022.827117. PMID: 35574105; PMCID: PMC9094064.Huraet al. (2022) Physiological and molecular features predispose native and invasive populations of sweet briar (Rosa rubiginosa L.) to colonization and restoration of drought degraded environments, Perspectives in Plant Ecology, Evolution and Systematics, Volume 56, 2022, 125690, ISSN 1433-8319, https://doi.org/10.1016/j.ppees.2022.125690.Loudyaet al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Bapatlaet al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Szymanskaet al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Rozpadeket al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Borovikand Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.Aswaniet al. (2018). Oxidative stress induced in chloroplasts or mitochondria promotes proline accumulation in leaves of pea (Pisum sativum): another example of chloroplast-mitochondria interactions. Protoplasma. 2018 Sep 11. doi: 10.1007/s00709-018-1306-1.Giovanardiet al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.Hanschenet al. (2018). Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiol Biochem. 2018 Mar;124:126-135. doi: 10.1016/j.plaphy.2018.01.009.Krasuskaet al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4