Goat anti-Rabbit IgG (H&L), TRITC conjugated

Goat anti-Rabbit IgG (H&L), TRITC conjugated

• Background: Goat anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethyl rhodamine-5-isothiocyanate) conjugatedis a secondary antibody which binds to rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.
• CAS Number: 9007-83-4
• Host: Goat
• Clonality: Polyclonal
• Dilution: The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
• Purity: Immunogen affinity purified IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
• Storage Conditions: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.