Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to bovine,goat,human, mouse,Rat IgG

• Background:

  Goat anti-rabbit IgG (H&L), DyLight® 550 conjugated,is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight® 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• Host:

  Goat

• Clonality:

  Polyclonal

• Dilution:

  1 : 50 -1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC),

• Purity:

  Immunogen affinity purified IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4