Anti-PsaD | PSI-D subunit of photosystem I
CAT:
451-AS09 461
Size:
50 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Anti-PsaD | PSI-D subunit of photosystem I
- Background: PsaD(PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.
- CAS Number: 9007-83-4
- Host: Rabbit
- Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi(red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays
- Not reactive in: Synechococcus elongatussp. PCC 7942
- Immunogen: KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences includingArabidopsis thalianaPSI-D1 UniProt:Q9S7H1, TAIR:At4g02770and PSI-D2 UniProt:Q9SA56, TAIRAt1g03130as well asPhyscomitrella patens. The conservation inChlamydomonas reinhardtiiis high (14 of 16 aminoacids are identical).
- Clonality: Polyclonal
- Applications: Clear-native PAGE (CN-PAGE), Immunoprecipitation (IP), Western blot (WB)
- Dilution: 1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)
- Purity: Serum
- Format: Lyophilized
- Reconstitution: For reconstitution add 50 µl of sterile water
- Molecular Weight: 17.9 | 20 (forArabidopsis thaliana)
- Precautions: This antibody is a replacement for former product, anti-PsaD AS04 046Contains 0.1% ProClin.
- References & Citations: Hanget al. (2024). HOT3/eIF5B1 confers Kozak motif-dependent translational control of photosynthesis-associated nuclear genes for chloroplast biogenesis. Nat Commun. 2024 Nov 14;15(1):9878. doi: 10.1038/s41467-024-54194-1.Penzleret al. (2024). A pgr5 suppressor screen uncovers two distinct suppression mechanisms and links cytochrome b6f complex stability to PGR5. Plant Cell. 2024 Mar 27:koae098. doi: 10.1093/plcell/koae098.Völkneret al. (2024). Evidence for partial functional overlap of KEA and MSL transport proteins in the chloroplast inner envelope of Arabidopsis thaliana. FEBS Lett. 2024 Aug;598(15):1877-1887. doi: 10.1002/1873-3468.14887.Uflewskiet al. (2024). The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status. Nat Commun. 2024 Mar 30;15(1):2792. doi: 10.1038/s41467-024-47151-5.Okegawaet al. (2023). x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light. Plant Physiol. 2023 Nov 22;193(4):2498-2512.doi: 10.1093/plphys/kiad466.Hao and Malnoë(2023). A Simple Sonication Method to Isolate the Chloroplast Lumen in Arabidopsis thaliana.Bio Protoc. 2023 Aug 5; 13(15): e4756.Jinet al. (2023) Dual roles for CND1 in maintenance of nuclear and chloroplast genome stability in plants. Cell Rep. 2023 Mar 28;42(3):112268. doi: 10.1016/j.celrep.2023.112268. Epub 2023 Mar 17.Maedaet al. (2022). Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana. J Plant Res. 2022 Mar;135(2):361-376. doi: 10.1007/s10265-022-01376-x. Epub 2022 Feb 10. PMID: 35146632.(CN-PAGE)GaoY et al. (2022) Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation. Plant Cell. 2022 Apr 26;34(5):2056-2079. doi: 10.1093/plcell/koac056. PMID: 35171295; PMCID: PMC9048916.Espinoza-Corral& Lundquist. (2022) The plastoglobule-localized protein AtABC1K6 is a Mn2+-dependent kinase necessary for timely transition to reproductive growth. J Biol Chem. 2022 Apr;298(4):101762. doi: 10.1016/j.jbc.2022.101762. Epub 2022 Feb 22. PMID: 35202657; PMCID: PMC8956952.Xionget al. (2022) a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana. Nucleic Acids Res. 2022 Jun 23;50(12):6715–34. doi: 10.1093/nar/gkac501. Epub ahead of print. PMID: 35736138; PMCID: PMC9262611.Ivanovet al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Fukuraet al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Fattoreet al. (2021). Acclimation of photosynthetic apparatus in the mesophilic red alga Dixoniella giordanoi. Physiol Plant. 2021 Nov;173(3):805-817. doi: 10.1111/ppl.13489. Epub 2021 Jul 5. PMID: 34171145; PMCID: PMC8596783.Chenet al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902.Pipitoneet al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Kameaet al. (2021). Substitution of deoxycholate with the amphiphilic polymer amphipol A8-35 improves the stability of large protein complexes during native electrophoresis. Plant Cell Physiol. 2021 Jan 5:pcaa165. doi: 10.1093/pcp/pcaa165. Epub ahead of print. PMID: 33399873.Asoet al. (2021). Unique peripheral antennas in the photosystems of the streptophyte alga Mesostigma viride. Plant Cell Physiol. 2021 Jan 8:pcaa172. doi: 10.1093/pcp/pcaa172. Epub ahead of print. PMID: 33416834.Tangel al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.Wanget al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .BN-PAGETeubneret al. (2020). The chloroplast ribonucleoprotein CP33B quantitatively binds the psbA mRNA. doi.org/10.1101/2020.02.11.944249Stortiet al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Xuet al. (2019). VENOSA4, a Human dNTPase SAMHD1 Homolog, Contributes to Chloroplast Development and Abiotic Stress Tolerance.Chenet al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Furukawaet al. (2019). Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens. J Plant Reshttps://doi.org/10.1007/s10265-019-01138-2.Stortiet al. (2018). Role of cyclic and pseudo-cyclic electron transport in response to dynamic light changes in Physcomitrella patens. Plant Cell Environ. 2018 Nov 29. doi: 10.1111/pce.13493.Maoet al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Namaet al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.Gaoet al. (2018). Effect of green light on the amount and activity of NDH-1–PSI supercomplex in Synechocystis sp. strain PCC 6803. Photosynthetica (2018) 56: 316.https://doi.org/10.1007/s11099-018-0790-z.Konget al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361Liet al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Duet al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.Schottleret al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Merryet al. (2017). A comparison of pine and spruce in recovery from winter stress; changes in recovery kinetics, and the abundance and phosphorylation status of photosynthetic proteins during winter. Tree Physiol. 2017 Sep 1;37(9):1239-1250. doi: 10.1093/treephys/tpx065.Geat al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.Yang-ErChen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Gerottoet al. (2016). Flavodiiron proteins act as safety valve for electrons in Physcomitrella patens. PNAS DOI 10.1073.Heinnickelet al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Fristedtet al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.Fujiiet al. (2015). Photoprotection vs Photoinhibition of Photosystem II in Transplastomic Lettuce (Lactuca sativa) Dominantly Accumulating Astaxanthin. Plant Cell Physiol. 2015 Dec 7. pii: pcv187.Daddyet al. (2015). A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803. Sci Rep. 2015 Mar 30;5:9480. doi: 10.1038/srep09480.Armbrusteret al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439Qinet al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.Chengand He (2014). PfsR Is a Key Regulator of Iron Homeostasis in Synechocystis PCC 6803. PLoS One. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743. eCollection 2014.Tomizioliet al. (2014). Deciphering thylakoid sub-compartments using a mass spectrometry-based approach. Mol Cell Proteomics. 2014 May 28. pii: mcp.M114.040923.Langet al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
- Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.