Chicken anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse IgG

• Background:

  Chicken anti-rabbit IgG (H&L)is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) inimmunological assays.

• Host:

  Chicken

• Reactivity:

  Rabbit IgG (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Rabbit IgG

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  The optimal working dilution should be determined by the investigator

• Purity:

  Immunogen affinity purified chicken IgY.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• Precautions:

  This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.

• References & Citations:

  Vergara-Cruces(2024). Structure of the plant plastid-encoded RNA polymerase. Cell . 2024 Feb 29;187(5):1145-1159.e21. doi: 10.1016/j.cell.2024.01.036.

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4