Chicken anti-Rabbit IgG (H&L), HRP conjugated

Chicken anti-Rabbit IgG (H&L), HRP conjugated

• Background: Chicken anti-rabbit IgG (H&L)is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) inimmunological assays.
• CAS Number: 9007-83-4
• Host: Chicken
• Reactivity: Rabbit IgG (H&L)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Rabbit IgG
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: The optimal working dilution should be determined by the investigator
• Purity: Immunogen affinity purified chicken IgY.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily
• Precautions: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
• References & Citations: Levitanet al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Gaoet al. (2018). Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2. Plant Biotech. J. 10.1111/pbi.13046
• Storage Conditions: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.