Chicken anti-Rabbit IgG (H&L), HRP conjugated

• Background:

  Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

• HS Code:

  38221900

• Host:

  Chicken

• Reactivity:

  Rabbit IgG (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Rabbit IgG

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Field of Research:

  Anti-Rabbit

• Dilution:

  The optimal working dilution should be determined by the investigator

• Purity:

  Immunogen affinity purified chicken IgY.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily

• Precautions:

  This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

• Additionnal Information:

  Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.2) HRP-conjugate is supplied in PBS, 1% BSA and 0.1% proclin 150.0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

• References & Citations:

  Levitan et al. (2019) . Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116 (35) :17316-17322. doi: 10.1073/pnas.1906726116.Gao et al. (2018) . Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2. Plant Biotech. J. 10.1111/pbi.13046

• Storage Conditions:

  Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• Prediction Reactivity:

  Rabbit IgG (H&L)

• CAS Number:

  9007-83-4