Chicken anti-Rabbit IgG (H&L), ALP conjugated

Chicken anti-Rabbit IgG (H&L), ALP conjugated

• Background: Chicken anti-rabbit IgG (H&L)is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG  (H&L) inimmunological assays.
• CAS Number: 9007-83-4
• Host: Chicken
• Reactivity: Rabbit IgG (H&L)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Rabbit IgG
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
• Purity: Immunogen affinity purified chicken IgY.
• Format: Liquid
• Precautions: This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
• Storage Conditions: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.