Anti-HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Anti-HDEL | Endoplasmic reticulum retention signal (clone 2E7)

• Background: HDEL(his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Oryza sativa, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
• Clonality: Monoclonal
• Applications: Western blot (WB), Immunofluorescence (IF), Immunogold (IG)
• Dilution: 1: 50 - 1: 500 (IF), 1 : 100 - 1: 1000 (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Liquid
• Molecular Weight: 78 kDa
• Precautions: Antibody in concentration 1 µg/mL was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992.
• References & Citations: Wanget al. (2023). OsCPK12 phosphorylates OsCATA and OsCATC to regulate H2O2 homeostasis and improve oxidative tolerance in rice. Plant Commun. 2023 Dec 21:100780.doi: 10.1016/j.xplc.2023.100780.Ming-fanget al. (2021) Improved quantification of immune-gold labeling and its use to compare the distribution of cellular factors among sub-chloroplast compartments,Micron,2021,103060,ISSN 0968-4328,https://doi.org/10.1016/j.micron.2021.103060.Luoet al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.Napieret al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
• Storage Conditions: Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.