Rabbit anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse and Rat IgG

• Background:

  Rabbit anti-goat IgGis a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins inimmunological assays.

• CAS Number:

  9007-83-4

• Host:

  Rabbit

• Reactivity:

  Goat IgG heavy and light chains (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Goat IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

• Purity:

  Immunogen affinity purified rabbit IgG.

• Format:

  Liquid

• Precautions:

  No reactivity is observed to non-immunoglobulin goat serum proteins based or IgG from human, mouse or rat based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.