Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

• Background:

  Goat anti-rabbit IgG (H&L),  HRP conjugated,is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Clonality:

  Polyclonal

• Dilution:

  1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

• Purity:

  Immunogen affinity purified IgG, F(ab)'2 fragment.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• References & Citations:

  Bandayand Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.