Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated

• Background:

  Rabbit anti-goat IgG (H&L),HRP conjugatedis a  F(ab)'2 fragment of a secondary antibody which binds to all goat IgGs in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

• Host:

  Rabbit

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  1 : 500-1 : 5000 (IHC),1 : 200 -1 : 5 000 (WB)

• Purity:

  Immunogen affinity purified IgG, F(ab)'2 fragment.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4