Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample

Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample

• Background: Rabbit anti-mouse IgGis a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins inimmunological assays.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Mouse IgG heavy and light chains (H&L)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Mouse IgG, whole molecule
• Clonality: Polyclonal
• Applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
• Purity: Immunogen affinity purified rabbit IgG.
• Format: Liquid
• Precautions: No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
• Storage Conditions: Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.