Rabbit anti-Mouse IgG (H&L), ALP conjugated

• Background:

  Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.

• HS Code:

  38221900

• Host:

  Rabbit

• Reactivity:

  Mouse IgG heavy and light chains (H&L)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Mouse IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)

• Field of Research:

  Anti-Mouse

• Dilution:

  1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

• Purity:

  Immunogen affinity purified rabbit IgG.

• Format:

  Liquid, clear, colorless.

• Precautions:

  Based on IEP, this antibody binds to:heavy chains on mouse IgGlight chains on all mouse immunoglobulinsNo reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

• Additionnal Information:

  ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4