Rabbit anti-Mouse IgG (H&L), HRP conjugated

Rabbit anti-Mouse IgG (H&L), HRP conjugated

• Background: Rabbit anti-mouse IgGis a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins inimmunological assays.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Mouse IgG heavy and light chains (H&L)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Mouse IgG, whole molecule
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
• Purity: Immunogen affinity purified rabbit IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
• Precautions: No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
• References & Citations: Vitaleet al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.Buiet al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.Kochet al. (2019). Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci U S A. 2019 May 21;116(21):10333-10338. doi: 10.1073/pnas.1820130116.
• Storage Conditions: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.