Anti-PsaL | PSI-L subunit of photosystem I

Anti-PsaL | PSI-L subunit of photosystem I

• Background: PsaL(PSI-L) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-L is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.In plants and algae Psa-L is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabaccum, Spinacia oleracea
• Not reactive in: Cyanobacteria
• Immunogen: KLH-conjugated synthetic peptide derived from PsaL protein sequence fromArabidopsis thaliana(At4g12800). This sequence is well conserved in most mono- and dicots but not inPhyscomitrella patens.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 18 | 17-18 (Arabidopsis thaliana)
• References & Citations: Wanget al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.Kohet al. (2019). Heterologous synthesis of chlorophyllÃ? bÃ? inÃ? Nannochloropsis salinaÃ? enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels.Ã? 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3. eCollection 2019.Schöttleret al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot.Ã? 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.SookSeok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.Bock(2012). The plastid genome-encodedYcf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.