Anti-PsbD | D2 protein of PSII

• Background:

  D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA) . PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39.5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.

• HS Code:

  38221900

• Gene ID:

  844775

• UniProt:

  P56761 , P11849 , P06007 , P20898

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Chromochloris zofingiensis, Echinola crus-galli, Emiliania huxleyi, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Skeletonema costatum (diatom), Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Triticale, Ulva prolifera, Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis thaliana P56761, Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

• Clonality:

  Polyclonal

• Applications:

  Clear-native PAGE (CN-PAGE), Western blot (WB)

• Field of Research:

  Global Antibodies

• Dilution:

  1: 10 000 (CN-PAGE), 1: 5000 - 1 : 50 000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  39.4 | 28-30 kDa

• Precautions:

  There is a confirmed cross-reaction with TLA1 protein inChlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

• Additionnal Information:

  The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower plants, cyanobacteria and unicellular algae except: minor substitutions in some Prochlorococcus & Dinoflagellate sequences, The antibody should still work against these taxa, but it has not been tested yet, This antibody does not detect PsbA protein (D1), This product can be sold containing ProClin if requested

• References & Citations:

  Li et al. (2025) . Salicylic acid and ROS signaling modulate hypocotyl elongation in darkness via NPR1 and EX1. Sci Adv. 2025 Oct 31;11 (44) :eadx4417. doi: 10.1126/sciadv.adx4417.Zhong et al. (2025) . Met246 and Asn250 in the D2 Protein are Essential for the Operation of the Quinone-Fe-Acceptor Complex of Photosystem II. Plant Cell Physiol. 2025 Jul 14:pcaf078. doi: 10.1093/pcp/pcaf078.Lang et al. (2025) . Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early photosystem II assembly factor. Plant Cell. 2025 Jun 4;37 (6) :koaf055. doi: 10.1093/plcell/koaf055.Krupinska et al. (2025) . Iron allocation to chloroplast proteins depends on the DNA-binding protein WHIRLY1. Planta. 2025 Jun 17;262 (2) :32. doi: 10.1007/s00425-025-04736-8. McKenzie and Puthiyaveetil (2025) . Protein phosphorylation and oxidative protein modification promote plant photosystem II disassembly for repair. Plant Commun . 2025 Mar 10;6 (3) :101202. doi: 10.1016/j.xplc.2024.101202.Zhao et al. (2024) . Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844. Ciesielska et al. (2024) . S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318. Khaig and Eaton-Rye (2023) . Lys264 of the D2 Protein Performs a Dual Role in Photosystem II Modifying Assembly and Electron Transfer through the Quinone–Iron Acceptor Complex. Biochemistry 2023, 62, 18, 2738–2750 Jiang et al. (2023) . Toxic effects of lanthanum (III) on photosynthetic performance of rice seedlings: Combined chlorophyll fluorescence, chloroplast structure and thylakoid membrane protein assessment. Ecotoxicol Environ Saf. 2023 Nov 15:267:115627.doi: 10.1016/j.ecoenv.2023.115627. Rredhi et al. (2023) . The UV-A Receptor CRY-DASH1 Up- and Downregulates Proteins Involved in Different Plastidial Pathways. J Mol Biol. 2023 Sep 10:168271.doi: 10.1016/j.jmb.2023.168271.Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022) . https://doi.org/10.1038/s41586-022-04662-9Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187 (4) :2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.von Bismarck et al. (2021) Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis. Research Square; 2021. DOI: 10.21203/rs.3.rs-948381/v1.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Aegilops tauschii, Brassica napus, Cannabis sativa, Capsicum annuum, Centrolepsis monogyna, Chromera velia, Crocosphaera watsonii, Cyanidioschyzon merolae, Fischerella sp., Galdieria sulphuraria, Glycine max, Glycine soja, Leiosporoceros dussii, Cucumis sativa, Manihot esculenta, Marchantia polymorpha, Microcystis aeruginosa, Nannochloropsis, Panax ginseng, Petermannia cirrosa, Pinus thunbergii, Physcomitrium patens, Pinus strobus, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Spinacia oleracea, Solanum lycopersicum. Triticum aestivum, Utricularia alpina, Vitis vinifera, Vitrella brassicaformisSpecies of your interest not listed? Contact us

• CAS Number:

  9007-83-4