Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

• Background: ATP synthaseis the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Camelina sinensis,Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Dionaea muscipula, Echinochloa crus/galli,  Escherichia coli, Gossypium hirsutum, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum,Moniliophthora perniciosa,Nannochloropsis salina,Neochloris oleoabundans(chlorophyta),Nicotiana bentamiana, Nicotiana tabacum,Oryza sp.(roots, leafs, pollen), Ostreococcus tauri,Pheodactylum tricornutum CCAP 1055/1,Pisum sativum,Plasmodium berghei,Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal,Locusta migratoria
• Not reactive in: Archeal V-type ATP synthase
• Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, includingArabidopsis thalianachloroplastic ATP synthase subunit beta UniProt:P19366, TAIR:AtCg00480andArabidopsis thalianamitochondrial ATP synthase subunit beta-1, UniProt:P83483,  TAIR:At5g08670as well asChlamydomonas reinhardtii,UniProt:P06541andA8IQU3
• Clonality: Polyclonal
• Applications: Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Ultrastructure Expansion Microscopy (U-ExM), Western blot (WB)
• Dilution: 1 : 100 (IF), 1 : 5000 (BN-PAGE), 1: 2500 (U-ExM), 1 : 2000-1 : 5 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (SynechocystisPCC 6803), 53.7 kDa (Spinacia oleracea)
• Precautions: Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria,Bacillus cereus.
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• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.