Anti-RpoB | RNA polymerase beta subunit (chloroplast)

Anti-RpoB | RNA polymerase beta subunit (chloroplast)

• Background: RpoB (RNA polymerase beta subunit (chloroplast))is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: His-tagged, highly conserved fragment ofZea maysRpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt:A0A059Q6W3
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 500 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 121 kDa
• Precautions: Antibody does not work on total cell extracts. Stromal fraction has to be used.This antibody is detecting recombinant RpoB.
• References & Citations: Vergara-Cruces(2024). Structure of the plant plastid-encoded RNA polymerase. Cell . 2024 Feb 29;187(5):1145-1159.e21. doi: 10.1016/j.cell.2024.01.036.Zhanget al. (2017). PDM3, a pentatricopeptide repeat-containing protein, affects chloroplast development. J Exp Bot. 2017 Nov 28;68(20):5615-5627. doi: 10.1093/jxb/erx360.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.