Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies)

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies)

• Background: ATP synthaseis the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
• CAS Number: 9007-83-4
• Host: Chicken
• Reactivity: Arabidopsis thaliana, Hordeum vulgare, Phaeodactylum tricornutum, Spartina alterniflora, Spinacia oleracea, SynechocystisPCC 6803,SynechococcusPCC 7942, beef muscle, Rat liver
• Not reactive in: Archeal V-type ATP synthase
• Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, includingArabidopsis thalianachloroplastic ATP synthase subunit beta UniProt:P19366, TAIR:AtCg00480andArabidopsis thalianamitochondrial ATP synthase subunit beta-1, UniProt:P83483,  TAIR:At5g08670as well asChlamydomonas reinhardtii,UniProt:P06541andA8IQU3
• Clonality: Polyclonal
• Applications: Immunolocalization (IL), Immunogold (IG), Western blot (WB)
• Dilution: 1 : 500 for localization of native enzyme by immunogold (IL), 1 : 5 000-1 : 8 000 (WB)
• Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
• Format: Liquid at 23.3 µg/µL
• Molecular Weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (SynechocystisPCC 6803), 53.7 kDa (Spinacia oleracea)
• Precautions: Results of immunogold studies using anti-AtpB antibody are published inAnderssonet al. (2009).
• References & Citations: Neusiuset al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Levitanet al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Nelsonet al. (2019). Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. doi: 10.1111/pce.13692.Gellertet al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005. (immunogold)Quesadaet al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13.Anderssonet. al (2009). Co-localization of P-glycerate kinase, P-ribulokinase, ADP-glucose pyrophosphorylase and Rubisco activase with CF1 in pea leaf chloroplasts. Plant Science 177:136-14. (immunologold).
• Storage Conditions: Store at 2-8°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.