Mouse anti CD68 (Macrosialin), conjugated to FITC

Mouse anti CD68 (Macrosialin), conjugated to FITC

• Background: CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located intracellularily in cytoplasmic granules but can also be detected in smaller amounts at the cell surface. Particular strong intracellular expression is observed for monocytes and macrophages. In addition, Langerhans cells as well as plasmacytoid dendritic cells express clear-cut levels of CD68. Low-level reactivity is also observed with a subset of B lymphocytes and activated T lymphocytes. Oxidized low-density lipoprotein is a ligand for CD68. The CD68 mAb permits the identification and enumeration of monocytes, Langerhans cells and plasmacytoid dendritic cells (in combination with CD4/CD56 staining) in normal and malignant Human blood and bone marrow samples using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
• CAS Number: 9007-83-4
• UniProt: P34810
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG1
• Clone: Ki-M7
• Conjugation: FITC
• Type: Primary Antibodies
• Applications: Flow Cytometry
• Purification Method: Purified by Chromatography
• Assay Principle: Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX,PERM? (Cat. No. GAS-002) intracellular CD68 can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µL of whole blood, bone marrow or mononuclear cell suspension in a 5mL tube - Add 100 µL of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5 mL phosphate buffered saline and centrifµge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100µL Reagent B (Permeabilization Medium) and 20 µL of the CD68 monoclonal antibody conjµgate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 mL 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze fixed cells within 24 hours
• Form: Purified by Chromatography, Storage buffer: PBS pH 7,2, 1% BSA, 0,05% NaN3
• Precautions: For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
• References & Citations: 1. Davey, F. R., Cordell, J. L., Erber, W. N., Pulford, K. A., Gatter, K. C. , Mason, D. Y. (1988) J Clin Pathol 41, 753-8. _x000D_ 2. Feuillard, J., Jacob, M. C., Valensi, F., Maynadie, M., Gressin, R., Chaperot, L., Arnoulet, C., Brignole-Baudouin, F., Drenou, B., Duchayne, E., Falkenrodt, A., Garand, R., Homolle, E., Husson, B., Kuhlein, E., Le Calvez, G., Sainty, D., Sotto, M. F., Trimoreau, F. , Bene, M. C. (2002) Blood 99, 1556-63._x000D_ 3. Fukuda, M. (1991) J Biol Chem 266, 21327-30. _x000D_ 4. Holness, C. L. , Simmons, D. L. (1993) Blood 81, 1607-13. _x000D_ 5. Knapp, W., Strobl, H. , Majdic, O. (1994) Cytometry 18, 187-98. _x000D_ 6. Pulford, K. A., Rigney, E. M., Micklem, K. J., Jones, M., Stross, W. P., Gatter, K. C. , Mason, D. Y. (1989) J Clin Pathol 42, 414-21. _x000D_ 7. Rabinowitz, S. S. , Gordon, S. (1991) J Exp Med 174, 827-36. _x000D_ 8. Ramprasad, M. P., Fischer, W., Witztum, J. L., Sambrano, G. R., Quehenberger, O. , Steinberg, D. (1995) Proc Natl Acad Sci U S A 92, 9580-4. _x000D_ 9. Ramprasad, M. P., Terpstra, V., Kondratenko, N., Quehenberger, O. , Steinberg, D. (1996) Proc Natl Acad Sci U S A 93, 14833-8. _x000D_ 10. Sadovnikova, E., Parovichnikova, E. N., Savchenko, V. G., Zabotina, T. , Stauss, H. J. (2002) Leukemia 16, 2019-26. _x000D_ 11. Scheinecker, C., Strobl, H., Fritsch, G., Csmarits, B., Krieger, O., Majdic, O. , Knapp, W. (1995) Blood 86, 4115-23. _x000D_ 12. Strobl, H. , Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9. _x000D_ 13. Strobl, H., Scheinecker, C., Csmarits, B., Majdic, O. , Knapp, W. (1995) Br J Haematol 90, 774-82. _x000D_ 14. Strobl, H., Scheinecker, C., Riedl, E., Csmarits, B., Bello-Fernandez, C., Pickl, W. F., Majdic, O. , Knapp, W. (1998) J Immunol 161, 740-8
• Storage Conditions: Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody; For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.