Anti-human CD45 PTPRC Monoclonal Antibody APC Conjugated, Flow Validated

• Background:

  CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP) . Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

• Description:

  Boster Bio Anti-human CD45 PTPRC Monoclonal Antibody APC Conjugated, Flow Validated (Catalog# FC00555-APC) . Tested in Flow Cytometry application (s) . This antibody reacts with Human.

• Synonyms:

  Receptor-type tyrosine-protein phosphatase C;3.1.3.48; Leukocyte common antigen; L-CA; T200; CD45; PTPRC; CD45

• Gene Name:

  PTPRC

• UniProt:

  P08575

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human T lymphocytes

• Clonality:

  Monoclonal

• Clone:

  Clone: Clone: F10-89-4

• Conjugation:

  APC

• Applications:

  Flow Cytometry

• Field of Research:

  Adapters, Adaptive Immunity, B Cells, Cell Type Markers, Immunology, Protein Phosphorylation, Signal Transduction, T Cells, Transmembrane, Tyrosine Phosphatases

• Purification:

  Protein A purified

• Sensitivity:

  5ul per sample

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  User needs to optimize the dilution ratio for this antibody.

• Form:

  Liquid

• Function:

  Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) . .

• Molecular Weight:

  147254 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 2-8°C. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG2a, k

• Other Gene Names:

  Receptor-type tyrosine-protein phosphatase C

• Subcellular Location:

  Membrane; Single-pass type I membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts.

• Protein Name:

  Receptor-type tyrosine-protein phosphatase C