IFN-g Interferon-Gamma Rat Recombinant Protein

• Description:

  IFN-gamma Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 135 amino acids and having a molecular mass of 15609 Dalton. The IFN-gamma is purified by proprietary chromatographic techniques.

• Synonyms:

  Immune IFN; type II IFN; T cell IFN; MAF; IFNG; IFG; IFI; IFN-gamma

• Gene Name:

  IFNG

• UniProt:

  P01581

• Reactivity:

  Rat

• Tissue Specificity:

  Released primarily from activated T lymphocytes.

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  The specific activity as determined by the cytopathic affect inhibition assay with murine L929 cells chalenged with EMC virus was less than 0.1 ng/ml, corresponding to a specific activity of 10,000,000units/mg.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized IFN-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  Interferon gamma

• Subcellular Location:

  Secreted.