Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®, Carrier-free

Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®, Carrier-free

• Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
• Description: Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (96 Tests). Quantitate Human IFNG in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Interferon gamma;IFN-gamma;Immune interferon;IFNG;
• Gene Name: Interferon gamma
• Gene ID: 3458
• UniProt: P01579
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-Q166
• Tissue Specificity: Released primarily from activated T lymphocytes.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <2 pg/ml
• Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 19348 MW
• Specificity: Natural and recombinant human IFN gamma
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	34	38	37	36	36	1.47	4%
  Sample 2	150	152	164	166	158	7.07	4.4%
  Sample 3	469	446	458	468	460	9.28	2%

  *number of samples for each test n=16.
• Subcellular Location: Secreted.
• Sequence Similarities: Belongs to the type II (or gamma) interferon family.
• Protein Name: Interferon gamma