Human MIP-1α/CCL3 PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.

• :

  heparin, EDTA or citrate

• Description:

  Human MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CCL3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	33	34	32	35	33	1.11	3.3%
  Sample 2	175	166	172	161	168	5.4	3.2%
  Sample 3	370	407	390	394	390	13.27	3.4%

  *number of samples for each test n=16.

• Synonyms:

  C-C motif chemokine ;CCL3 ;hCG_1749202 ;

• Gene Name:

  C-C motif chemokine

• Gene ID:

  6348

• UniProt:

  A0N0R1

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: A27-A92

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  10085 MW

• Specificity:

  Natural and recombinant human MIP-1 alpha

• Protein Name:

  C-C motif chemokine 3