Rat MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

• :

  heparin, EDTA or Citrate

• Description:

  Rat MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	18	18	18	16	17	0.86	5%
  Sample 2	54	52	47	47	50	3.08	6.1%
  Sample 3	267	269	269	304	277	15.46	5.5%

  *number of samples for each test n=16.

• Synonyms:

  C-C motif chemokine 3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3;Ccl3;Mip1a, Scya3;

• Gene Name:

  C-C motif chemokine 3

• Gene ID:

  25542

• UniProt:

  P50229

• Reactivity:

  Rat

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: A24-A92

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

• Components:

  Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

• Storage Conditions:

  Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

• Calculated Molecular Weight:

  10335 MW

• Specificity:

  Natural and recombinant rat Ccl3

• Subcellular Location:

  Secreted.

• Protein Name:

  C-C motif chemokine 3