Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®, Carrier-free

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®, Carrier-free

• Background: Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 ly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
• Description: Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 3;Heparin-binding chemotaxis protein;L2G25B;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;SIS-alpha;Small-inducible cytokine A3;TY-5;Ccl3;Mip1a, Scya3;
• Gene Name: C-C motif chemokine 3
• Gene ID: 20302
• UniProt: P10855
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-A92
• Tissue Specificity: Expressed in lung, spleen, and pancreas.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 7.8 pg/ml - 500 pg/ml
• Sensitivity: <10 pg/ml
• Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse CCL3
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	18	19	17	17	17	0.82	4.8%
  Sample 2	74	69	76	69	72	3.08	4.2%
  Sample 3	240	225	228	207	225	11.81	5.2%

  *number of samples for each test n=16.
• : heparin, EDTA or citrate
• Calculated Molecular Weight: 10345 MW
• Subcellular Location: Secreted.
• Protein Name: C-C motif chemokine 3