Human Complement Factor H/CFH ELISA Kit PicoKine®

Human Complement Factor H/CFH ELISA Kit PicoKine®

• Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
• Description: Human Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests). Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Complement factor H;H factor 1;CFH;HF, HF1, HF2;
• CAS Number: 7732-18-5
• Gene Name: Complement factor H
• Gene ID: 3075
• UniProt: P08603
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Target protein isolated from human plasma
• Tissue Specificity: Expressed by the liver and secreted in plasma.
• Applications: ELISA
• Standard: 100 ng/tube
• Sensitivity: <50 pg/ml
• Function: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
• : heparin or EDTA
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
• Detection Range: 1.56 ng/ml - 100 ng/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 139096 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	3203	2994	3170	3050	3104	85.4	2.7%
  Sample 2	13518	12944	13467	11714	12910	726.54	5.6%
  Sample 3	53844	53833	53094	52680	53362	497.77	0.9%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant human CFH
• Subcellular Location: Secreted.
• Protein Name: Complement factor H