Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine®, Carrier-free

• Background:

  Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18q11. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.

• :

  heparin, EDTA or citrate

• Description:

  Rat SEMA6A ELISA Kit PicoKine® (96 Tests). Quantitate Rat Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	88	83	88	77	84	4.52	5.3%
  Sample 2	461	388	441	444	433	27.35	6.3%
  Sample 3	1323	1468	1499	1453	1435	67.17	4.6%

  *number of samples for each test n=16.

• Synonyms:

  Semaphorin 6A; Sema6a

• Gene Name:

  semaphorin 6A

• Gene ID:

  361324

• UniProt:

  D3ZAG0

• Reactivity:

  Rat

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G19-T649

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant rat SEMA6A

• Subcellular Location:

  Extracellular space. Integral component of plasma membrane.